Table 2.

Half-lives of RS cocultured with Sertoli cells in the absence or presence of TGFβ1

Treatment	Half-life (days)
Control	1.9 ± 0.2
TGFβ1	1.9 ± 0.2

Elutriated RS were cocultured with Sertoli cells for 5 days in the absence (control) or presence of 10 ng/ml TGFβ1 and the number of remaining RS was assessed by flow cytometry daily between day 2 and day 5 allowing the determination of their half-life in culture. Each value is the mean ± SEM of three different experiments.