Table 4.

Numbers of metaphase I and metaphase II on different days of coculture of PS and Sertoli cellsin the absence or presence of TGFβ1

	Number of metaphases I/mm			Number of metaphases II/mm
Days of coculture	Control		TGFβ1	Control		TGFβ1
1	0.09 ± 0.02	NT	0.03 ± 0.01	1.8 ± 0.3	NS	1.8 ± 0.3
3	0.15 ± 0.04	NT	0.05 ± 0.03	2.3 ± 0.3	p < 0.01	3.1 ± 0.4
5	0.04 ± 0.02	NT	0.03 ± 0.01	0.7 ± 0.1	p < 0.05	0.9 ± 0.2

PS were cocultured for 5 days in the absence (control) or presence of 10 ng/ml TGFβ1. On days 1, 3 and 5, the numbers of metaphase I and of metaphase II were determined by microscopic examination of the culture wells and expressed as the number of each type per mm of well and on the width of the microscope field (objective × 100). 400 to 1700 metaphase II were counted in each condition, whereas the number of metaphase I which could be counted ranged between 15 and 75 precluding meaningful statistical analysis.

Each value is the mean ± SEM of 5 different experiments.

NS : not significant ; NT : not tested.