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. 2005 Jun 21;33(11):3550–3560. doi: 10.1093/nar/gki657

Figure 1.

Figure 1

RP-HPLC profiles of d(GPS(FMA)CPS(FMA)TPS(FMA)APS(FMA)GPS(FMA)APS(FMA)CPS(FMA)GPS(FMA)TPS(FMATPS(FMA)APS(FMA)GPS(FMA)CPS(FMA)GPS(FMA)T) [CpG ODN fma1555]. Magenta line: Chromatographic profile of purified CpG ODN fma1555. Brown line: Chromatographic profile of purified CpG ODN fma1555 that was heated in 1× PBS (pH 7.2) for 626 h at 37°C. Turquoise line: Chromatographic profile of purified CpG ODN fma1555 that was heated in 1× PBS (pH 7.2) at 37°C for 73 h. RP-HPLC analyses were performed using a 5 μm Supelcosil LC-18S column (4.6 mm × 25 cm) according to the following conditions: starting from 0.1 M TEAA (pH 7.0), a linear gradient of 1% MeCN/min is pumped at a flow rate of 1 ml/min for 40 min. Peak heights of each profile are normalized to the highest peak, which is set to one arbitrary unit.