FIG. 2.

Suppression of eIF5-CTD Ts⁻ mutations by eIF2 TC overexpression. Two independent transformants of indicated strains carrying an empty vector YEplac195 (Vec.) or p1780-IMT (eIF2 TC) were grown in synthetic dextrose (SD) medium supplemented with tryptophan and diluted to the same density. Five microliters of the diluted culture was spotted onto SD plates containing tryptophan and incubated at the indicated temperatures for 2 days. Doubling time (d.t.) in the liquid medium was measured for two independent transformants, and the average values are indicated beside the growth patterns. N. T., not tested.