TABLE 4.
Analysis of whole bone marrow from SSB-2−/− and wild-type mice as determined by colony assay formation in response to cytokine stimulationa
| Mouse type and stimulusb | No. of colonies formed
|
|||||
|---|---|---|---|---|---|---|
| B | G | GM | M | Eo | Meg | |
| WT | ||||||
| GM-CSF | 17.5 | 10 | 22 | 1.5 | ||
| G-CSF | 11 | 0 | 0 | |||
| M-CSF | 3 | 4.5 | 58.5 | |||
| IL-3 | 5 | 15.5 | 11.5 | 13 | 1 | 7 |
| EPO | 8 | |||||
| SCF | 3.5 | 13.5 | 1 | 1 | 0 | |
| TPO | 2.15 | |||||
| SCF + IL-3 + EPO | 5.5 | 15.5 | 15 | 16.5 | 0.5 | 25 |
| IL-6 | 8.5 | 0.5 | 1.5 | 2 | ||
| KO | ||||||
| GM-CSF | 12.5 | 3.5 | 25 | 1.5 | ||
| G-CSF | 5.5 | 0.5 | 0 | |||
| M-CSF | 1.5 | 3 | 44 | |||
| IL-3 | 3.5 | 11.5 | 12 | 15.5 | 0.5 | 6 |
| EPO | 4.5 | |||||
| SCF | 4 | 12 | 0.5 | 0.5 | 0 | |
| TPO | 2 | |||||
| SCF + IL-3 + EPO | 7 | 17.5 | 9.5 | 13.5 | 0.5 | 18.5 |
| IL-6 | 7.5 | 0 | 0.5 | |||
a
A total of 25,000 cells in agar culture were stimulated by the cytokines listed, and colonies were enumerated. Colonies were identified as blast (B), granulocyte (G), granulocyte/macrophage (GM), macrophage (M), eosinophil (Eo), or megakaryocyte (Meg). Values are averages from two independent experiments.
b
See Table 3, footnote b. KO, knockout; WT, wild type.