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srs2-7AV and srs2-hd mutants are defective in DNA damage-induced subcomplexes formation. Crude protein extracts prepared from srs2-7AV (CY6005) and srs2-hd (CY6002) strains grown in unperturbed conditions (A) or in the presence of MMS (B) were fractionated by gel filtration and analyzed by Western blotting using antibodies against Srs2, Sgs1, and Mre11. Gel filtration analysis performed on the wild-type strain, taken from Fig. 2, is also shown.
