FIG. 7.

Model for the redistribution of Srs2, Sgs1, and Mre11 in DNA damage-specific complexes. Srs2, Sgs1, and Mre11 are involved in the formation of complex A under unperturbed conditions. A fraction of Srs2 is also found in cell cycle-dependent complex C*. In response to DNA damage and checkpoint activation, Sgs1 and Mre11 form complex B, while Srs2 and Mre11 form complex D. The phosphorylated Srs2 isoform localizes mainly at complex C. Cdk1-induced Srs2 phosphorylation and Tel1 activity allow the assembly of complexes B and D.