TABLE 1.
Average numbers of SSU processomes and cleaved transcripts in Esf2p depletion and control conditions
| Condition (no. of genes analyzed) | Avg value (range)b
|
||||
|---|---|---|---|---|---|
| No. of SSU processomes/gene | Position of first SSU processomec | No. of transcripts in last half of gene | No. of cleaved transcripts | % of cleaved transcripts in second half of gene | |
| Control (11) | 9.6 (3-18) | 52.3 (40-65) | 28 (17-40) | 8.1 (4-20) | 29 (14-50) |
| Depletion time (h)a | |||||
| 10 (15) | 9.7 (5-16) | 48 (40-75) | 20.6 (12-31) | 7.4 (1-19) | 36 (6-76) |
| 18 (17) | 7.9 (0-26) | 53.8 (20-75) | 24 (13-39) | 2.1 (0-5) | 8.6 (0-21) |
| 24 (15) | 6.1 (1-18) | 64 (50-100) | 18.2 (10-23) | 1.7 (0-4) | 9.5 (0-27) |
a
Depleted strains were grown in 10 μg/ml doxycycline for the number of hours indicated.
b
The percentage of cleaved transcripts at each time point was evaluated with Student's t test. Results for the control and 10-hour depletion were not significantly different from each other, and neither were those for the 18-hour and 24-hour depletions. All other paired combinations were significantly different, with P values of 0.0002 or lower.
c
Percent distance along gene from 5′.