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. 2005 Jul;25(13):5648–5663. doi: 10.1128/MCB.25.13.5648-5663.2005

FIG. 3.

FIG. 3.

Priming produces changes at the chromatin level on the promoter of TGM2. (A) DNase I sensitivity of the RARβ promoter in naive and primed cells. DNase I hypersensitivity was measured as described in Materials and Methods. Values are the means of three independent QPCR measurements ± standard deviations of a representative experiment. (B) DNase I sensitivity of the TGM2 promoter in naive and primed cells. DNase I hypersensitivity was measured as described in Materials and Methods. Values are the means of three independent QPCR measurements ± standard deviations of a representative experiment. (C) Chromatin immunoprecipitation analysis of HR1 enhancer element of TGM2. H4 acetylation was measured as described in Materials and Methods. The acetylation level is expressed as a percentage of input DNA. All no-antibody controls were lower than 0.2% of input DNA. Values are the means of three independent QPCR measurements of a representative experiment. Chromatin immunoprecipitation results were confirmed in at least three independent chromatin preps.