Table 1.

The primers sequences of the qRT-PCR in human and mouse colonic tissue

Species	Primer	Primer	Sequences (5'-3')
Human	PD-1	Forward	5’-CGTGGCCTATCCACTCCTCA-3’
		Reverse	5’-ATCCCTTGTCCCAGCCACTC-3’
	PD-L1	Forward	5’-TATGGTGGTGCCGACTACAA-3’
		Reverse	5’-TGGCTCCCAGAATTACCAAG-3’
	GAPDH	Forward	5’-TGATGACATCAAGAAGGTGGTGAA-3’
		Reverse	5’-TCCTTGGAGGCCATGTGGGCCAT-3’
Mouse	IL-1β	Forward	5'-GAGCCCATCCTCTGTGACTC-3'
		Reverse	5'-TCCATTGAGGTGGAGAGCTT-3'
	IL-6	Forward	5’-CCGGAGAGGAGACTTCACAG-3’
		Reverse	5’-TCCACGATTTCCCAGAGAAC-3’
	IL-17	Forward	5'-TCCCTCTGTGATCTGGGAAG-3'
		Reverse	5'-CTCGACCCTGAAAGTGAAGG-3'
	PD-1	Forward	5’-AAATCGAGGAGAGCCCTGGA-3’
		Reverse	5’-CATGCCTTGAAACCGGCCTT-3’
	PD-L1	Forward	5’-GGTGCGGACTACAAGCGAAT-3’
		Reverse	5’-TTCATGCTCAGAAGTGGCTGG-3’
	GAPDH	Forward	5’-TGATGACATCAAGAAGGTGGTGAAG-3’
		Reverse	5’-TCCTTGGAGGCCATGTGGGCCAT-3’

qRT-PCR, quantitative real-time polymerase chain reaction.