FIG. 1.

Representative selection of Piccolo deletion complexes used for HAT assays. The Coomassie-stained SDS-PAGE gel shows recombinant Piccolo complexes coexpressed in E. coli and purified by Talon chromatography. Constituent polypeptides in each deletion are described in Fig. 2. Piccolo v3 in lane 1 was additionally purified by Source Q and S chromatography. Epl1, Yng2, and Esa1 polypeptides for each deletion are indicated with a black square, black triangle, and open circle, respectively, to the right of the appropriate band. An E. coli contaminant with apparent size of 43 kDa that copurifies over Talon chromatography is labeled with a double-headed arrow between lanes 2 and 3. Molecular size standards are shown in lane 18 with corresponding sizes to the right (in kilodaltons).