FIG. 2.

Deletion analysis of Piccolo subunits. The chart shows the identity of subunits present in each Piccolo deletion construct, with evolutionarily conserved regions (EPcA, PHD, and chromodomain), structurally determined regions (Esa1 HAT domain), and hexahistidine tags shown as black and shaded regions. Naked histone and nucleosome HAT activities were normalized by ELISA-determined Esa1 content using anti-Esa1 antibodies. The preference for nucleosome ratio shown on the right side is the ratio of naked histone HAT activity to nucleosome HAT activity, with Piccolo v7 set to 1.00.