FIG. 4.

Specific mutations in Esa1 chromodomain severely affect growth of yeast cells. Yeast strains deleted for Esa1 and containing a wild-type Esa1 gene on a low-copy-number URA3 plasmid were transformed with a LEU2 plasmid expressing wild-type Esa1 or Esa1 containing the indicated point mutations in the chromodomain. The left panels show growth of 10-fold serial dilutions on YPD rich medium, while the right panels show similar growth on 0.1% fluoroorotic acid plates. Rows 6 and 8 document the severe growth defects of yeast cells expressing the Y56A and E65L Esa1 chromodomain mutations.