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. 2005 Jul;25(13):5675–5686. doi: 10.1128/MCB.25.13.5675-5686.2005

FIG. 2.

FIG. 2.

Interaction between HIF-1α and HIF-2α and effects on cyclin D1 expression. (A) RNase protection assay of HIF-1α, HIF-2α, and CCND1 mRNAs (U6 snRNA, loading control) in RCC4 cells after treatment for 48 h with Oligofectamine alone (−), HIF-1α-directed siRNA (H1), HIF-2α-directed siRNA (H2), or control siRNA (C). (B and D) Immunoblots, with the indicated antibodies, of whole-cell lysates from RCC4 and SKRC28 cells that were infected with retroviral supernatants made from pLZRS containing GFP alone (G), HIF-1α (H1), or HIF-2α (H2). (C and E) Immunoblots for HIF-1α, HIF-2α, and β-tubulin of RCC4 and SKRC28 whole-cell lysates after treatment for 48 h with a control siRNA (C), HIF-1α siRNA (H1), or HIF-2α siRNA (H2). (F) Immunoblots for HIF-1α, HIF-2α, and β-tubulin of RCC4 whole-cell lysates treated with the previously indicated siRNAs and then exposed to 1 mM MMOG for 18 h. Two independent experiments for each condition are shown in panels B, C, D, E, and F.