Fig. 4.
The role of RSV glycoprotein during infection in vivo. Purified murine alveolar macrophages were stimulated with RSV F protein (closed bar), RSV glycoprotein from antigenic subgroup A (GA protein, open bar) or RSV F and glycoproteinA (horizontal stripped bar) (a); or RSV (dark gray bar), ΔG (vertical stripped bar), or GΔ172–187 (black horizontal stripped bar) (b). (c) Virus titration in lungs of mice 4 days after infection. Viruses were inoculated intranasally at 106 pfu (n = 5 per group). (d) Intracellular expression of IL-6 by alveolar macrophages from mice infected with RSV, ΔG, or placebo analyzed by flow cytometry 24 h after infection. Viruses were inoculated intranasally at 106 pfu. (e) Pulmonary histopathology score assessing PMN and macrophage infiltration after infection with RSV (filled squares), ΔG (open squares), or mG (gray squares). (f) Pulmonary histopathology in mice 24 h after infection with the indicated virus (PAS, ×10).