Figure 5. ET-1R activation enhances the number, distance and size of invasive bodies.

(A) Model of invasion patterns near the ACM. (B) Number of invasive bodies and (C) distance of invasion into stromal compartment after drug treatment with ET-1 (100 nM) and/or macitentan (MAC) (1 µM) by day 14. (D) Example of surface area of invasive bodies for the ET-1 treatment and control group, showing a 3-fold up-regulation after ET-1 treatment. Invasive bodies were stained for CK7 (red) and DAPI (blue). Scale bar = 100 µm. Significance signifies ordinary one-way ANOVA P value with Dunnett's multiple comparison test.