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. 2005 Jun 13;102(25):8905–8909. doi: 10.1073/pnas.0501034102

Fig. 2.

Fig. 2.

Dynamics of DNA methyltransferase recruitment to repair sites in living cells. Live cell imaging of microirradiated C2C12 cells in S phase coexpressing fluorescent fusion constructs of DNA methyltransferases and PCNA is shown. The constructs used were the same as depicted in Fig. 1 A except for the RFP-Dnmt1 construct where the GFP was replaced by monomeric RFP. Maximum projections of confocal midsections are shown and times after microirradiation are indicated. (A) A C2C12 cell in early to mid S phase coexpressing GFP-Dnmt1 and RFP-PCNA shows accumulation of RFP-PCNA and GFP-Dnmt1 at sites of DNA damage (arrows) as early as 1 min after irradiation. (B) An early S phase cell coexpressing GFP-Dnmt3a and RFP-Dnmt1 shows accumulation of RFP-Dnmt1 at the irradiated site (arrow), whereas GFP-Dnmt3a is bleached (arrow) and does not recover during the entire observation period. (C) An early S phase cell coexpressing GFP-Dnmt3b1 and RFP-PCNA shows bleaching of GFP-Dnmt3b1 (arrow) at the irradiation spot, whereas RFP-PCNA accumulates at this site (arrow). (Scale bars, 5 μm.)