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Recruitment of GFP-Dnmt1 and RFP-PCNA to repair sites in S and non-S phase C2C12 cells. The structure of the fusion proteins is depicted in Fig. 1 A. Live cell imaging of microirradiated C2C12 cells in G1 (A) and late S phase (B) coexpressing GFP-Dnmt1 and RFP-PCNA shows accumulation of RFP-PCNA and GFP-Dnmt1 at sites of DNA damage (arrows). Maximum projections of confocal midsections are shown and times after microirradiation are indicated. (Scale bars, 5 μm.)
