Fig. 4.

Srx1 expression depends on Sty1. (A) Northern blot analysis of srx1 expression. Total RNA from strains HM123 (WT), AM004 (Δpap1), AM001 (Δsty1), and JM1066 (Δatf1) was obtained from cultures treated with H₂O₂ for the times and concentrations indicated. (B and C) The late oxidation (B) and nuclear accumulation (C) of Pap1 after 1 mM H₂O₂ stress depends on Sty1 and Srx1. The redox state (with nonreducing SDS/PAGE followed by Western blot analysis) and cellular distribution (with fluorescence microscopy) of a GFP-Pap1 fusion protein was determined in cells treated with H₂O₂ for the concentrations and times indicated. The GFP-Pap1-expressing strains used in this experiment were EHH14 (WT), EA36 (Δsrx1), AV1 (Δsty1) and AV1 overexpressing Srx1 from plasmid p120.42x (psrx1.42x).