Table 1. Nonviremic transmission of WNV between Cx. p. quinquefasciatus mosquitoes.
Days p.i. of donor mosquitoes (experiment) | Infectious blood meal virus titer/ml | Mean donor whole body virus titer* | No. of donors engorged | No. of recipients infected/n (%)† | Mean recipient whole body titer (n)† |
---|---|---|---|---|---|
7 (1a) | 7.95 | 5.49 ± 0.88 | 48 | 2/36 (5.6) | 4.29 ± 4.57 (2) |
14 (1b) | 7.95 | 5.67 ± 1.66 | 39 | 10/173 (5.8) | 4.55 ± 2.27 (10) |
14 (2) | 8.52 | 6.70 ± 0.62 | 7 | 1/50 (2.0) | 4.52 ± 0.00 (1) |
14 (3) | 8.95 | 6.09 ± 0.55 | 57 | 3/124 (2.4) | 5.47 ± 0.50 (3) |
14 (4) | 7.95 | 5.95 | 1 | 2/87 (2.3) | 4.74 ± 0.30 (2) |
All titers are expressed as tissue culture infectious dose endpoints (log10 TCID50 per ml or per mosquito).
Mean whole body titer based on TCID50 of 20 donor mosquitoes with blood in gut from cofeed (except for experiments 2 and 4, which had only 7 and 1 infected mosquitoes engorged on the mouse, respectively).
Recipients tested on day 14 subsequent to cofeeding with donors.