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. 2024 Sep 2;44(11):1231–1260. doi: 10.1002/cac2.12607

FIGURE 3.

FIGURE 3

SRSF10 promoted M2 macrophage polarization through LA. (A) qRT‐PCR detected the expression of CD206, ARG1, IL10, and ADM mRNA in THP1 cells co‐cultured with Huh7 cells from shCTRL, shSRSF10, and shSRSF10 with LA groups (n = 3). (B) qRT‐PCR detected the expression of Cd206, Arg1, Vegfa, and Adm mRNA in BMDMs co‐cultured with Hepa1‐6 cells from shCtrl, shSrsf10, and shSrsf10 with LA groups (n = 3). (C) Expression of CD206 in THP1 cells, detected by flow cytometry analyses (n = 3). (D) Expression of Cd206 in BMDMs, detected by flow cytometry analyses (n = 3). (E) Chemotactic migration assays and statistical analysis of macrophages stimulated with the supernatant of Huh7 or Hepa1‐6 cells (n = 3). (F) Western blotting analysis shows the levels of Pan Kla in THP1 cells and BMDMs cultured with control or shSRSF10 HCC cells. (G‐I) Western blotting analysis shows the levels of Pan Kla and H3K18la in THP1 cells and BMDMs. (J‐K) qChIP analysis of the indicated promoters was performed using antibodies against H3K18la (n = 3). Two‐tailed unpaired Student's t test (A‐E, J‐K); ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001. 2‐DG, 2‐Deoxy‐D‐glucose; ADM, adrenomedullin; ARG1, arginase 1; BMDMs, Bone‐marrow‐derived macrophage; CD206, CD206 molecule; ChIP, Chromatin immunoprecipitation; DMEM, Dulbecco's Modified Eagle Medium; IL10, interleukin 10; LA, lactic acid; OE, over expression; qRT‐PCR, Quantitative Real‐Time Reverse Transcription Polymerase Chain Reaction; SRSF10, serine and arginine rich splicing factor 10; Vegfa, vascular endothelial growth factor A.