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. Author manuscript; available in PMC: 2024 Nov 18.
Published in final edited form as: J Biomed Mater Res A. 2022 Jan 25;110(6):1190–1198. doi: 10.1002/jbm.a.37362

FIGURE 1.

FIGURE 1

Extracellular vesicle (EV) characterization and bioactivity assessment. (A) Nanotracking analysis of mesenchymal stem/stromal cell (MSC) EVs. (B) Immunoblots of EV-associated markers CD63, ALIX, TSG101, and Flotillin-1 as well as endoplasmic reticulum (ER) marker (Calnexin). (C, D) Human umbilical vein endothelial cells (HUVECs) were treated with growth media only (EGM; positive control), basal media only (EBM; negative control), 100 μg/ml MSC EVs in basal media. Images were taken at time 0 (brightfield) and 12 h post wounding of the cell layer. Representative images for each group and timepoint are displayed and the gap area outlined with a white line. Statistical significance was calculated using one-way ANOVA with Holm–Sidak’s test (**p < .01, ***p < .001) (n = 3)