Fig. 3.

Time series of single-molecule fluorescence images of Dronpa in PVA (pH 7.4). A and B denote two different molecules. The images were obtained with a 488-nm excitation at a power of 220 nW. The integration time was 1 ms/pixel with the pixel size of 78 × 78 nm. The same area was scanned with a 405-nm laser at a power of 220 nW (1 ms/pixel with the pixel size of 78 × 78 nm) before scanning images 5, 9, 13, and 17 with a 488-nm laser.