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. 2005 Jun 22;102(27):9511–9516. doi: 10.1073/pnas.0500489102

Fig. 4.

Fig. 4.

Photoswitching of Dronpa at the single-molecule level. (a) Fluorescence intensity trajectory from a single Dronpa molecule in PVA (pH 7.4) on two-color excitation at a power of 220 and 37 nW at 488 and 405 nm, respectively. Lower shows the on–off state of the 488-nm (cyan line) and 405-nm (blue line) light. (b and c) Zoom of the bursts in the trajectory. (d–f) Frequency histogram of on-time (d), medium-long off-time (e), and short off-time (f). Solid lines in d and f show a single-exponential fitting. Solid line in f shows a G fit. (g) Fluorescence intensity trajectory from a single Dronpa molecule in PVA (pH 7.4) on two-color excitation at a power of 320 and 37 nW at 488 and 405 nm, respectively. Lower shows the on–off state of the 488-nm (cyan line) and 405-nm (blue line) light.