Force-displacement relation in response to circumferential (circ, black stripes) and longitudinal (long, white stripes) stretch is increased in C57BL/6NTac mice in the presence of AVP. In ZYM-treated BL/6J (teal bars) and BL/6NTac (orange bars) mice, the force-displacement relation was measured before and after AVP application in the bath solution. Data are presented as AUC and peak responses to stretch during AVP application relative to the baseline measures. (A) A 2 (Strain) × 2 (Stretch Direction) ANOVA revealed a significant main effect of Strain (F [1, 15] = 30.810, P < .001) and a Strain × Stretch Direction interaction (F [1, 15] = 4.787, P < .05) on AUC. (B) Similarly, 2 × 2 ANOVA for force-displacement during peak stretch force application confirmed a significant main effect of Strain (F [1, 15] = 57.203, P < .001), confirming a greater response magnitude in BL/6NTac mice treated with ZYM compared with BL/6J mice treated with ZYM though this did not differ for the 2 stretch directions. No other significant main effects or interactions were observed (all P > .05) (n = 4–6). * indicates significant strain x stretch direction interaction based on ANOVA. ** indicates significant main effect of strain.