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. Author manuscript; available in PMC: 2024 Nov 18.
Published in final edited form as: Nat Protoc. 2019 Oct 4;14(11):3059–3081. doi: 10.1038/s41596-019-0212-0

Fig. 8 |. Detection of DNA methylation in Bdnf, S1pr2 and S1pr1 promoter regions in microvessels and whole brain.

Fig. 8 |

Methylation status was examined using methylation-specific PCR with specific primers located in Bdnf promoter IX and S1pr1 and S1pr2 core promoter regions. Genomic DNA was isolated from whole-brain (WB) and the microvessel fraction of adult brain (MV; 2 months old, C57BL/6J) and subjected to bisulfite treatment. The PCR products were analyzed on a 2% (wt/vol) agarose gel. Left lane:100-bp DNA ladder.