Table 1 |.

Troubleshooting table

Step	Problem	Possible reason	Solution
5	Delayed or improper dissection of cortices	Usage of inappropriate tools can cause difficulties	Use curved-end forceps
11	The pellet does not seem red or is covered with a white layer	The pellet was not properly resuspended	Mix the pellet from Step 9 completely with 15% (wt/vol) dextran–DPBS by pipetting
	The pellet does not appear at the bottom of tube	The concentration of dextran solution was incorrect	Confirm that the dextran completely dissolves. Do not exceed the correct final volume
12	Debris is too close to the pellet. It is difficult to get a clean pellet	Dextran was not mixed thoroughly with brain lysate or a small volume of dextran solution was used	Increase the volume of dextran and mix the pellet thoroughly by pipetting. If necessary, wipe out debris from the walls of the tube with a Kimwipe
14	The pellet becomes trapped inside the pipette tip	The microvessels can easily adhere to the surface of plastic	Pre-wet the pipette tip by pipetting 1× DPBS in and out repeatedly
17	The pellet is very small or invisible	The microvessels remain in the cell strainer or BSA was not added to the medium	Use enough volume to retrieve the microvessels from the strainer. Add BSA (0.5% (wt/vol)) to pellet the microvessels