Table 2 |.
Primer sequences
| Gene | Sequence (5′–3′) |
|---|---|
|
| |
| Primers used for gene expression analysis by qPCR | |
| Tjp1 (ZO1) F | CCA AGG TCA CAC TGG TGA AGT C |
| Tjp1 (ZO1) R | CTT GAA TGT TAC CAT CTC TTG CTG |
| Aqp4 F | TGG TTG GAG GAT TGG GAG TCA C |
| Aqp4 R | CAG TTC GTT TGG AAT CAC AGC TG |
| Anpep (CD13) F | CAA GGG AGC CTC AGT CAT CAG GAT GC |
| Anpep (CD13) R | GAC AGC TGT CTG TTG GTT CAC G |
| S1pr2 F | ATGGGCGGCTTATACTCAGAG |
| S1pr2 R | GCGCAGCACAAGATGATGAT |
| S1pr2 pre-mRNA F | GCC ATA ACT GTG ACC TGC TGT C |
| S1pr2 pre-mRNA R | TCT CAG GAT TGA GGT ACT CTG AG |
| Gfap F | GCTGGAGGGCGAAGAAAAC |
| Gfap R | TGGCCTTCTGACACGGATTT |
| Sele (E-selectin) F | CCGTCCCTTGGTAGTTGCA |
| Sele (E-selectin) R | CAAGTAGAGCAATGAGGACGATGT |
| Pecam1 (CD31) F | GCG GTG TTC AGC GAG ATC CTG |
| Pecam1 (CD31) R | ACT GTA CCT CGT TAC TCG ACA G |
| Primers used for DNA methylation analysis by PCR | |
| BDNF promoter IX, Chr2: 109722792–109722873 (GeneBank: GRCm38.p4 C57BL/6J) | |
| Methylation-specific primer F | TCGTAAAATATGGTGGTTTATATCGT |
| Methylation-specific primer R | AATATAATAAAACAACGATCGATCGA |
| Unmethylation-specific primer F | TTGTAAAATATGGTGGTTTATATTGT |
| Unmethylation-specific primer R | CAATATAATAAAACAACAATCAATCAA |
| S1PR2 promoter, Chr9: 20976033–20976175 (GeneBank: GRCm38.p4 C57BL/6J) | |
| Methylation--specific primer F | TGTTACGGACGAGCGAGCGG |
| Methylation-specific primer R | CTT CGA TAC AAA ATAA TTCTTCCGA |
| Unmethylation-specific primer F | AGGTGTTATGGATGAGTGAGTGG |
| Unmethylation-specific primer R | TCC TTC AAT ACAAAATAATTCTTCCAA |
| S1PR1 promoter, Chr3: 115714705–115714785 (GenBank: GRCm38.p4 C57BL/6J) | |
| Methylation-specific primer F | GTGAGTTGGTCGTCGTTAGTCG |
| Methylation-specific primer R | CTACCTCGCTCAAACCGAACG |
| Unmethylation-specific primer F | GAGTGAGTTGGTTGTTGTTAGTTG |
| Unmethylation-specific primer R | CCTACCTCACTCAAACCAAACAA |
F, forward; R, reverse.