Fig. 1.
Pixel-perfect multidomain image registration of A. thaliana. A) The central image displays a montage of the registered output data of three different sensor systems: an RGB sensor, a hyperspectral imaging sensor (HSI) and a chlorophyll fluorescence camera (ChlF). B-E) Frist row shows the segmented RGB, NDVIHSI, and Fv/Fm data and the grayscale of ChlF intensity. The images in the side column display data analysis by vegetation indices such as F) the anthocyanin reflectance index (ARI1), G) the chlorophyll: carotinoid index (CCI), H) the normalized difference vegetation index (NDVI) or calculated parameters from ChlF, such as I) non-photochemica quenching (NPQ). Images of A. thaliana of a salt treated variant (50 mM NaCl) and cultivated in the modified PhenoWell® culture system were taken at 21 days after treatment (DAT). Image registration was performed via NCC-adaptive approach in which a transformation matrix was derived by transforming the green channel of the RGB camera and the mean intensity from 540–560 nm of the HSI camera to the far-red reflectance (730 nm) of the ChlF image sensor as the target image. The white scale bar indicates 10 mm