Table 6.
Frame selection effect to image registration of RGB to ChlF on test data of A. thaliana
| Moving/ Traget image |
Metric | Blue Channel (~ 440 nm) |
Green Channel (~ 550 nm) |
Red Channel (~ 700 nm) |
Composite gray image |
|---|---|---|---|---|---|
|
Red reflection (660 nm) |
NCC [-] | 0.87 ± 0.02 | 0.90 ± 0.03 | 0.91 ± 0.03 | 0.91 ± 0.03 |
| ΔMP [px] | 4.85 ± 2.57 | 4.89 ± 2.51 | 4.83 ± 2.57 | 4.96 ± 2.48 | |
| OR [%] | 89.5 ± 3.1 | 89.9 ± 3.2 | 88.9 ± 3.5 | 89.5 ± 3.4 | |
| ΔMPConvex [px] | 2.46 ± 1.54 | 2.05 ± 1.65 | 2.06 ± 1.71 | 2.23 ± 1.59 | |
| ORConvex [%] | 95.8 ± 1.8 | 96.0 ± 1.8 | 95.6 ± 2.0 | 95.8 ± 1.9 | |
|
Far red reflection (730 nm) |
NCC [-] | 0.71 ± 0.07 | 0.86 ± 0.02 | 0.85 ± 0.03 | 0.84 ± 0.03 |
| ΔMP [px] | 9.99 ± 14.59 | 4.81 ± 2.17 | 4.91 ± 2.23 | 4.91 ± 2.25 | |
| OR [%] | 81.6 ± 21.5 | 92.6 ± 2.9 | 91.4 ± 3.5 | 92.0 ± 3.1 | |
| ΔMPConvex [px] | 7.55 ± 14.98 | 2.26 ± 1.57 | 2.22 ± 1.54 | 2.35 ± 1.51 | |
| ORConvex [%] | 88.8 ± 18.5 | 97.1 ± 1.6 | 96.6 ± 1.8 | 96.9 ± 1.6 | |
|
Chlorophyll fluorescence (> 650 nm) |
NCC [-] | 0.21 ± 0.05 | 0.31 ± 0.07 | 0.26 ± 0.07 | 0.27 ± 0.07 |
| ΔMP [px] | 107.25 ± 69.39 | 4.78 ± 2.44 | 47.33 ± 64.60 | 84.83 ± 123.26 | |
| OR [%] | 7.4 ± 4.4 | 93.6 ± 2.8 | 58.9 ± 44.4 | 58.2 ± 45.5 | |
| ΔMPConvex [px] | 108.57 ± 67.06 | 2.38 ± 1.65 | 45.53 ± 63.45 | 84.27 ± 126.42 | |
| ORConvex [%] | 12.0 ± 8.2 | 97.6 ± 1.6 | 62.5 ± 44.6 | 61.4 ± 46.5 |
Note: Image metrics were investigated with NCC-adaptive approach and ChlF as reference registration target. Mean ± SD