Figure 3.

Predicted structures of Sis1 interacting with oRPs. (A) AlphaFold Multimer structure of Sis1 dimers with oRPs depicted above the region of the ribosome containing each mature ribosomal protein (Evans et al., 2021, Preprint). These RPs are each integrated into the ribosome at different assembly steps. Sis1 dimers shown in gray; oRPs shown in magenta; rRNA shown in blue. The regions predicted to interact with Sis1, circled in green, are buried in rRNA in the mature ribosome. Importantly, these structures represent only single poses predicted by the model. These are almost certainly not the only way in which Sis1 can interact with these proteins. (B) Schematic of a single oRP. (C) Schematic for how RPs bind in a mutually exclusive manner to Sis1 and rRNA.