Fig. 10.

Xenotransplantation-induced angiogenesis in the zebrafish embryos. The alkaline phosphatase assay was performed to assess the angiogenic potential of two different cancer cell lines, A MDA-MB-231 and B MDA-MB-453, in embryos that were either untreated or treated, with enlargement of the sub-intestinal venous plexus (SIVP) region at 72 hpf (dorsal and lateral view). Magnification 32x. C The scheme depicts the counting of ectopic sprouts. D To present the results, a graph was generated showing the average number of SIVP branches at 72 hpf. Data are representative of three replicates (n = 30 for each group) and are shown as the mean ± standard deviation. ****p < 0.0001 in one-way ANOVA followed by Tukey’s test