Fig. 9.

Imaging of cancer xenografts and micrometastases in zebrafish acquired by digital light sheet microscopy. A Representative image of the yolk sac and tail regions were captured at 24 h and 48 h after microinjection of MDA-MB-231 breast cancer (BC) cells labeled in red. B Additionally, images were taken at 24 h after microinjection of MDA-MB-453 BC (red). Arrows were used to indicate the presence of tumor masses, micrometastases in the tail, as well as the formation of clusters of cancer cells (n = 30 for each group)