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. 2024 Oct 7;12(11):e04269-23. doi: 10.1128/spectrum.04269-23

Fig 10.

Four microscopy images showing brightfield or fluorescent images of calcofluor white stained yeast cells and VHH9 binding pattern. VHH9 binds to new buds.

Immunolabeling of C. albicans yeast with VHH9. Yeast cells of C. albicans strain SC5314 were grown in YPD medium overnight at 30°C and fixed with formaldehyde. The samples were stained with calcofluor white and labeled with VHH9, and a rabbit anti-VHH polyclonal antibody and a goat anti-Rabbit IgG–Alexa Fluor 647–conjugated antibody for detection. Panels indicate: (a) DIC, (b) calcofluor white, (c) Alexa Fluor 647, and (d) merged view. Cells were imaged using a Zeiss Axio Imager M2 microscope.