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. 2024 Oct 7;12(11):e04269-23. doi: 10.1128/spectrum.04269-23

Fig 12.

Four microscopy images showing brightfield or fluorescent images of calcofluor white stained yeast cells and VHH2 binding to discrete spots at the poles of the yeast cells.

Immunolabeling of C. albicans yeast with VHH2. Yeast cells of C. albicans strain SC5314 were grown in YPD medium overnight at 30°C and fixed with formaldehyde. The samples were stained with calcofluor white and labeled with VHH2, and a rabbit anti-VHH polyclonal antibody and a goat anti-Rabbit IgG–Alexa Fluor 647–conjugated antibody for detection. Panels indicate: (a) DIC; (b) calcofluor white; (c) Alexa Fluor 647; and (d) merged view. Cells were imaged using a Zeiss Axio Imager M2 microscope.