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. 2024 Oct 7;12(11):e04269-23. doi: 10.1128/spectrum.04269-23

Fig 6.

Four microscopy images depict cells under different conditions: brightfield, colored fluorescence, and a merged image combining the channels. Merged image highlights cells with overlapping fluorescence signals.

Immunolabeling of C. albicans yeast with VHH19. Yeast cells of C. albicans strain SC5314 were grown in YPD medium overnight at 30°C and fixed with formaldehyde. The samples were stained with calcofluor white and labeled with VHH19, and a rabbit anti-VHH polyclonal antibody and a goat anti-Rabbit IgG–Alexa Fluor 647–conjugated antibody for detection. Panels indicate: (a) DIC, (b) calcofluor white, (c) Alexa Fluor 647, and (d) merged view. Cells were imaged using a Zeiss Axio Imager M2 microscope.