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. 2024 Oct 7;12(11):e04269-23. doi: 10.1128/spectrum.04269-23

Fig 7.

Four microscopy images of hyphae: brightfield, colored fluorescence (blue for chitin binding, red for VHH binding) and a merged image combining channels. VHH19 binding is observed on mother bud and neck region of hyphae.

Immunolabeling of C. albicans hyphae with VHH19. Hyphae of C. albicans strain SC5314 were grown in RPMI-1640 medium at 37°C for 6 h and fixed with formaldehyde. The samples were stained with calcofluor white and labeled with VHH19, and a rabbit anti-VHH polyclonal antibody and a goat anti-Rabbit IgG–Alexa Fluor 647–conjugated antibody for detection. Panels indicate: (a) DIC, (b) calcofluor white, (c) Alexa Fluor 647, and (d) merged view. Cells were imaged using a Zeiss Axio Imager M2 microscope.