Skip to main content
. 2024 Aug 14;110(11):6941–6952. doi: 10.1097/JS9.0000000000002021

Figure 2.

Figure 2

In experiments A–C, 30 min prior to the onset of stress, mice were administered with propranolol (A), RU486 (B), or naltrexone (C), or with vehicle. In experiment D, mice were adrenalectomized (ADX) or underwent sham surgery 4 weeks before stress. In all four experiments, mice were subjected not to 8 h of hypothermic wet-cage (HWC) stress and administered with CpG-C or vehicle 2 h after the onset of stress. IL-12 plasma levels were assessed at the end of stress. CpG-C significantly elevated plasma IL-12 levels (A, n=57, n=6–9 per group; B, n=32, n=4 per group; C, n=75, n=8–10 per group, P<0.0001; D, n=91, n=11–14, P<0.0001) and wet-cage markedly attenuated CpG-C-induced increase in plasma IL-12 (A, P<0.0001; B, P<0.0001; C, P<0.0001; and D, P<0.0001). Adrenalectomy improved CpG-C-induced IL-12 levels, but only under HCC conditions (****P<0.0001). Boxes represent the second and third quartiles, and whiskers show min and max values.