Figure 5.

Comparison between hypothermic and normothermic conditions on CpG-C-induced IL-12 production. PBMCs were incubated for 10 or 20 h, at 33°C or 37°C. The study was conducted in duplicates, and supernatants were collected in duplicates from each well at the end of each incubation period. Hypothermic conditions significantly decreased (main effect, P<0.0001) the production of IL-12 by PBMCs, and CpG-C elevated it (main effect, P<0.0001). Hypothermic conditions attenuated CpG-C-induced elevation of IL-12 at 10 h (P<0.0001) and at 20 h (P<0.0001) (****P<0.0001). The graph represents mean±SEM.