FIGURE 7.

Effect of point mutation over the stability of p53 gene. (A) Structure of p53 with dsDNA (pdb id.: 2AC0), with stop codon readthrough derived mutations marked (Ma et al., 2018). (B) Change in the structural stability over the p53 protein upon inserting point mutations. (C) Conformational dynamics of p53 DNA binding domain (DBD) upon inserting point mutations (S127, P128, A129 with W/C/R mutations) derived from stop codon readthrough. Changes in the dStability (ΔStability) upon inserting mutations were traced using the residue scan pipeline implemented in the Molecular Operating Environment (MOE; Chemical Computing Group Inc., Montreal, QC, Canada) package. BIOVIA Discovery Studio visualizer (Dassault Systèmes, BIOVIA Corp., San Diego, CA, USA) package was used to visualise protein structures.