Figure 1.

Heterozygous knockout of Vstm2a promotes CRC development in an AOM/DSS mouse model

(A) Schematic diagram of integration site of vector used to generate Vstm2a knockout mice. (B) Representative images of the electrophoresis patterns of amplicon products from Vstm2a^+/− and wild-type mice as determined by genotyping PCR. (C) Representative image of IHC staining of Vstm2a in the colon of Vstm2a^+/− knockout mice and wild-type littermates. (D) Representative image of colon histology from Vstm2a^+/− mice and wild-type littermates. (E) Schematic diagram of experimental design for AOM/DSS-induced mouse CRC model. (F) Representative images of colon from Vstm2a^+/− mice and wild-type littermates at the end of experiment. (G) Tumor number and tumor load in Vstm2a^+/− mice and wild-type littermates. (H) Body weight of Vstm2a^+/− mice and wild-type littermates at the time of sacrifice. AOM, azoxymethane; DSS, dextran sodium sulfate.