Figure 2.

Heterozygous knockout of Vstm2a suppresses CD8⁺ cell infiltration

(A) Representative images of colon histology from Vstm2a^+/− mice and wild-type littermates. Histological features of the high-grade dysplasia and adenocarcinoma generated from Vstm2a^+/− mice are shown in the amplified images on the bottom. (B) Representative IHC images of colon tissues from Vstm2a^+/− mice and wild-type littermates stained with CD8a antibody. (C) Representative IHC images of colon lymph nodules from Vstm2a^+/− mice and wild-type littermates stained with CD8a antibody. (D) Statistical analysis of CD8a IHC staining in mice colon and lymph node. Vstm2a^+/− mice (n = 12); wild-type littermates (n = 15). (E) Flow cytometry of CD8⁺ T cell population in Vstm2a^+/− (n = 4) and wild-type littermates (n = 9). Each dot represents an independent mouse. (F) Gross tumor imaging of C57BL/6 mice subcutaneously implanted with MC38-expressing control or VSTM2A plasmid. n = 5 mice per group. (G) Size and weight of xenograft tumors. (H) Flow cytometry analysis of CD45⁺CD3⁺ tumor-infiltrated T cells in xenografts expressing VSTM2A or control vector.