Figure 6.

VSTM2A promotes IFN-γ secretion by PBMCs

(A) Images of the ELISpot assay showing IFN-γ secretion by PBMCs following 24 h stimulation with the anti-CD3 antibody or VSTM2A recombinant protein (1 μg/mL). (B) Summary of the IFN-γ spot number generated in (A). (C) Images of the ELISpot assay showing the IFN-γ secretion by DC:CD8⁺ T cell co-culture following 24 h stimulation with the anti-CD3 antibody or VSTM2A. Autologous DCs and CD8⁺ T cells were used in the analysis. (D) Summary of the IFN-γ spot number generated in (C). Statistical analysis was performed against Actin peptide using an unpaired t test. Data are presented as mean values ± SD.