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X-band EPR spectra of apo-NifDK proteins. Perpendicular mode EPR spectra of apo-NifDKRS and apo-NifDKAv in the DTH reduced states. Apo-NifDKAv was purified from a ΔnifH ΔnifB A. vinelandii strain. Pure apo-NifDKRS preparations were obtained from E. coli cells coexpressing NifHRS and either NifUSAv or a plasmid-cloned iscEc gene cluster. Apo-NifDKRS (IscEc) spectrum was obtained from 10 scans; apo-NifDKRS (NifUSAv) and apo-NifDKAv spectra were obtained from 5 scans.
