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Temperature dependence of cofactor synthesis and apo-NifDKRS reconstitution. Temperature screening was performed in the presence (red) or absence (blue) of molybdate. Reaction mixtures contained NifHRS, NifBRS, apo-NifDKRS, NifXAv, homocitrate, SAM, Fe, S, Mo (when indicated), and an ATP regenerating mixture (Materials and Methods). Negative control assays lacking NifBRS showed negligible activity. Data represent average activity ± SD (n = 2).
