Table 1.
Primers for qRT-PCR Used in the Current Study
| Primer | Sequence | |
|---|---|---|
| ICAM-1 | Forward (5ʹ-3ʹ) | TGGAGACGCAGAGGACCTTAAC |
| Reverse (5ʹ-3ʹ) | CGACGCCGCTCAGAAGAAC | |
| IRF7 | Forward (5ʹ-3ʹ) | TGAGCGAAGAGAGCGAAGAGG |
| Reverse (5ʹ-3ʹ) | CGTACACCTTATGCGGATCAACTG | |
| IL-6 | Forward (5ʹ-3ʹ) | GAGAGGAGACTTCACAGAGGATACC |
| Reverse (5´-3´) | TCATTTCCACGATTTCCCAGAGAAC | |
| IL-1β | Forward (5´-3´) | AATCTCGCAGCAGCACATCAAC |
| Reverse (5ʹ-3ʹ) | AGGTCCACGGGAAAGACACAG | |
| CCL2 | Forward (5´-3´) | ACTGCATCTGCCCTAAGGTCTTC |
| Reverse (5ʹ-3ʹ) | TCACTGTCACACTGGTCACTCC | |
| SOCS3 | Forward (5ʹ-3ʹ) | TCTTTGCCACCCACGGAACC |
| Reverse (5ʹ-3ʹ) | TCAGTACCAGCGGAATCTTCTCG | |
| β-actin | Forward (5ʹ-3ʹ) | TATGCTCTCCCTCACGCCATCC |
| Reverse (5ʹ-3ʹ) | GTCACGCACGATTTCCCTCTCAG |
Abbreviations: SAE, sepsis-associated encephalopathy (SAE); UTI, Ulinastatin; GEO, gene expression omnibus; DEGs, differentially expressed genes; IRGs, immune-related genes; DE-IRGs, differential expressed immune-related genes; GSEA, Gene set enrichment analysis; PPI, protein-protein interaction; Tregs, regulatory T cells; CLP, Cecal ligation and puncture; HE, hematoxylin eosin; MWM, Morris Water Maze; PCA, principal component analysis; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; BP, biological process; CC, cellular components; MF, molecular functions; MCC, maximal clique centrality; MNC, maximum neighborhood component; EPC, edge percolated component; GGI, gene-gene interaction; TFs, transcription factors; Dgidb, Drug–Gene Interaction Database (DGIdb); qRT-PCR, quantitative real-time polymerase chain reaction; DG, dentate gyrus; TNF, tumor necrosis factor; BBB, Blood-brain barrier; TLR4, Toll-like receptor 4.