Figure 6.

Contiune.

Figure 6.

The frequency of circulating IL-17-secreting CD4⁺ and CD8⁺ T cells positive for TLR-2 and TLR-4 as a function of relapse occurrence in MS patients. Taking into account the representative dot-plots and histograms shown in panel A showing activated (PMA plus ionomycin), or not (none), cells, the mean proportion of CD4⁺ and CD8⁺ T cells positive for TLR-2 (B) and TLR-4 (C), as well as MFI of TLR-2 (D) and TLR-4 (E) for cells in samples obtained from DMT-treated RRMS patients who relapsed (Relp, n=24) or not (Stab n=16) during follow-up (1 year) was determined by cytometry. The mean frequency of circulating IL-17-secreting cells among (F) TLR-2⁺ and (G) TLR-4⁺ (CD4⁺ and CD8⁺) T lymphocytes was evaluated after activation with PMA plus ionomycin. Data are shown as mean ± SD of eight independent experiments with 4 to 5 samples per experiment. Significance was calculated by comparing non-relapse (Ctrl) versus relapse (Relp) patients using one-way ANOVA. The p values are indicated in the figure.