Table 6.
Correlation Between Plasma LBP and Cytokine Profile in TLR-Stimulated T Cell Cultures from RRMS Patients
| Cytokines (pg/mL) | LBP (µg/mL) | |||
|---|---|---|---|---|
| Pam3C | LPS | |||
| r | p | r | p | |
| CD4+T cells | ||||
| IL-1β | 0.5566 | 0.0088 | 0.6590 | 0.0012 |
| IL-6 | 0.4737 | 0.0312 | 0.4252 | 0.0415 |
| TNF-α | 0.3840 | 0.0811 | 0.3132 | 0.1209 |
| GM-CSF | 0.7694 | <0.0001 | 0.6679 | 0.0009 |
| IL-17 | 0.4325 | 0.0378 | 0.4652 | 0.0354 |
| IL-21 | 0.2902 | 0.2019 | 0.3740 | 0.0944 |
| IL-22 | 0.2207 | 0.3363 | 0.3190 | 0.1211 |
| IFN-γ | 0.3781 | 0.0991 | 0.4820 | 0.0269 |
| IL-10 | 0.1099 | 0.6788 | 0.1254 | 0.5879 |
| CD8+ T cells | ||||
| IL-1β | 0.5100 | 0.0273 | 0.4533 | 0.0391 |
| IL-6 | 0.5081 | 0.0187 | 0.4452 | 0.0336 |
| TNF-α | 0.4155 | 0.0610 | 0.2681 | 0.2402 |
| GM-CSF | 0.3091 | 0.21244 | 0.1199 | 0.6048 |
| IL-17 | 0.5081 | 0.0184 | 0.4335 | 0.0407 |
| IL-21 | 0.1092 | 0.7691 | 0.1142 | 0.6642 |
| IL-22 | 0.2521 | 0.3360 | 0.3227 | 0.1537 |
| IFN-γ | 0.4307 | 0.0580 | 0.5419 | 0.0130 |
| IL-10 | 0.2133 | 0.4561 | 0.2786 | 0.3120 |
Note: The plasma concentration of LPS-binding protein (LBP) from RRMS patients [control (n= 14) and relapsed (n=7)], assayed through ELISA, were correlated with cytokine release by CD4+ and CD8+ T cell cultures stimulated with TLR-2 (Pam3C) and TLR-4 (LPS) agonist quantified by Multiplex. In bold, the significant values of correlation (p<0.05) are highlighted.