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. 2024 Nov 18;14:28518. doi: 10.1038/s41598-024-79312-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 13 — (A) Immune cells were counted in fifty-micron swaths of demineralized spines at the base, in the middle, and at the tip, and the percent of these counted cells in each region was calculated. (B) Velocity of individual presumptive red-spherule cells in intact spines from 5 spines of different Lytechinus variegatus individuals. Each dot on the graph represents an individual cell’s average velocity over the course of the time-lapse video. Significant p values between spines by One-way ANOVA are indicated with stars (*= 0.0282, **= 0.0023, ****= < 0.0001).