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. 2024 Nov 19;15(11):844. doi: 10.1038/s41419-024-07245-w

Fig. 3. MMP12 functions as an effector molecule in the process of RON-mediated bladder cancer cell migration and matrix invasion.

Fig. 3

A J82 cells were transduced with siRNA targeting MMP12 and an overexpression plasmid for RON. Western blot analysis was utilized to confirm the expression of MMP12. B The migratory capacity was assessed through a wound-healing assay. C The invasive potential was evaluated using a trans-well assay. D In total, 5637 cells were transduced with an overexpression plasmid for MMP12 and a shRON plasmid. Western blot analysis was employed to validate the expression of MMP12. E The migratory ability was assessed through a wound-healing assay. F The invasion ability was evaluated by trans-well assay. The scale bar above is all 1 µm. Actin-Tracker Red-555 and DAPI were utilized for staining the cytoskeleton and nucleus, respectively, with representative images displayed in Fig. (G) and (H). The scale bar measures 500 µm. I, J Western blot analysis was used to confirm the expression of N-cadherin and vimentin. All experiments above were repeated three times (n = 3).